A case of liver abscess with desulfovibrio desulfuricans. Aminoacidsequence of the cytochromec3 mr26000 from desulfovibrio desulfuricans norway and a comparison with those of the other polyhemic cytochromes from desulfovibrio. The two most common species of these bacteria, known scientifically as desulfovibrio desulfuricans or desulfotomaculum nigrificans, can be the cause of severe pitting corrosion even on stainless steel components. Desulfovibrio longus desulfovibrio piger desulfovibrio longreachensis desulfovibrio oxamicus desulfovibrio multispirans. Desulfovibrio desulfuricans isolates from the gut of a single. The authors have examined extracellular ironbearing precipitates resulting from the growth of desulfovibrio desulfuricans in a basal medium with lactate as the carbon source and ferrous sulfate.
Since these bacteria are killed by exposure to atmospheric oxygen, the environmental niches most frequently occupied. Desulfovibrio choose one desulfovibrio desulfuricans strain atcc 27774 dsm 6949 desulfovibrio desulfuricans dsm 642 desulfovibrio desulfuricans nd2 all lower taxonomy nodes 3 common name i. The product of this respiration, sulfide, is highly toxic and corrosive and is responsible for the environmental, economic, and health impact of these bacteria in their habitats. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers like other sulfatereducing bacteria, desulfovibrio was long considered to. Sulfate respiration in desulfovibrio vulgaris hildenborough. Under continuous culture conditions two effects of. Wall, university of missouri gerrit voordouw, university of calgary. Preferential reduction of the thermodynamically less favorable. The role of rnf in ion gradient formation in desulfovibrio. Recently, different studies demonstrated that lipopolysaccharides lps from gut bacteria and their.
The sulfate reducing bacterium desulfovibrio desulfuricans inhabits both. While most isolates have not been identified to the species level, desulfovibrio fairfieldensis and d. Reclassification of the only species of the genus desulfomonas. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. Desulfovibrio desulfuricans strain nd 2 was isolated from chesapeake bay sediments in may 1985 gilmour et al. The primary and threedimensional structures of a ninehaem. Here, we report the draft genome of the gramnegative, sulfatereducing bacterium desulfovibrio desulfuricans strain g11. Coordinated response of the desulfovibrio desulfuricans 27774. The toxicity of al to desulfovibrio desulfuricans g20 was assessed over a period of 8 weeks in a modi. Desulfovibrio desulfuricans isolates from the gut of a. The sulfatereducing bacterium desulfovibrio desulfuricans strain csn dsm 104 oxidized h 2 with thiosulfate, sulfate, sulfite, nitrite, nitrate and oxygen with rates increasing in the order listed from 20 to 525 nmol h 2 min. The primary and threedimensional structures of a nine. The sulfatereducing bacterium aldehyde oxidoreductase from desulfovibrio gigas mop is a member of the xanthine oxidase family of enzymes.
Nitrate reduction was induced by nitrate or limiting concentrations of sulfate during growth. Both positive anaerobic blood cultures were further subcultured on columbia blood agar with 5% horse blood and on chocolate agar. Desulfovibrio desulfuricans survived exposure to air on the surface of agar plates for at least 48 h. Desulfovibrio vulgaris an overview sciencedirect topics. Desulfovibrio cells have mesophillic growth patterns with an ideal growth rate between the temperatures of twentyfive to fourty degrees celcius ncbi. Atp sulfurylase from desulfovibrio desulfuricans atcc 27774 native. Although error bars resulting from combining data from independent experiments designed in. Sulfatereducing bacterium desulfovibrio desulfuricans nd2 as. Imbalances in the composition of gut microbiota have been reported to be associated with susceptibility to obesity and insulin resistance diseases 1, 2. Structural and biological lipid a characterization wei zhangsun equipe endotoxins. Threedimensional structure of the nonaheme cytochromec from desulfovibrio desulfuricans essex in the feiii state at 1. The disposal of elemental mercury hg0 wastes in mining and manufacturing areas has caused serious soil and groundwater contamination issues. According to a previous study 5 we expected that disproportionation is catalyzed by thi osulfate reductase and some of the enzymes involved in sulfate.
Its cells are gramnegative, slightly curved rods gilmour et al. Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells. The nature of the diiron site in the bacterioferritin from desulfovibrio desulfuricans article pdf available in nature structural biology 104. Dde i from desulfovibrio desulfuricans, strain norway. All lower taxonomy nodes 3 common name i synonym iother names i bacillus desulfuricans beijerinck 1895 saltet 1900 microspira desulfuricans beijerinck 1895 migula. Pdf crystallization and preliminary xray analysis of a. Desulfovibrio desulfuricans desulfuricans beijerinck, 1895 kluyver and van niel, 1936. Search thousands of articles and collaborate with scientists around the globe. Desulfovibrio desulfuricans strain 27774 is one of a relative small group of.
Desulfovibrio vulgaris, desulfovibrio desulfuricans, and desulfotomaculum. It is particularly noted for its ability to produce methyl mercury. Desulfovibrio desulfuricans was grown on a medium with an excess of pyruvate in relation to sulphate, so that the pyruvate could be dissimilated both by respiration and by fermentation. Desulfovibrio desulfuricans aestuarii postgate and campbell, 1966 subspecies. Barrett and sinclair, 1967 is autoxidizable but does not combine with cn or co. Desulfovibrio desulfuricans is a gramnegative sulfatereducing bacteria. Under aerobic conditions at 37c with 5% co2, no growth was obtained. Moreover, pdnaps could be synthesized by desulfovibrio desulfuricans. Haemcontaining proteins are directly involved in electron transfer as well as in enzymatic functions. Accumulation was not influenced by incubation in nacl instead of kcl, by acidic ph 5. Under aerobic conditions at 37c with 5% co 2, no growth was obtained. Chemolithotrophic growth of desulfovibrio desulfuricans. Desulfovibrio desulfuricans atcc 541 was obtained from the american type.
Complete genome sequence of desulfovibrio desulfuricans. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers. The reported case of a desulfovibrio desulfuricans bacteremia occurring in an immunocompromised host with ulcerative colitis confirms that this organism may be a possible opportunistic human. Desulfovibrio is known as a sulfate reducing bacterium, which has put it to the forefront of biological research. This name appeared on the approved lists of bacterial names 1980. Desulfovibrio africanus desulfovibrio desulfuricans desulfovibrio fructosovorans desulfovibrio gigas desulfovibrio salexigens desulfovibrio vulgaris desulfovibrio sp. Feio1, vitaly zinkevich1, enric llobetbrossa2, peter eaton1, jurgen schmitt3, jean guezennec4 7 and iwona b. Threedimensional structure of the nonaheme cytochrome c from. Models for the primary and threedimensional structures of this. Characterization of sulfate transport in desulfovibrio.
Pdf biogasenergy is marginally profitable against the parasitic energy demands of processing biomass. Discussion desulfovibrio desulfuricans has a constitutive thio sulfate reductase like most sulfatereducing bacteria and a constitutive capacity of thiosulfate dispropor tionation 5. Desulfovibrio desulfuricans atcc 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. Most desulfovibrio can tolerate small amounts of exposure to air. Negativeion maldi mass spectra of intact srb 1 and srb 2 lipid a, respectively, are presented on fig. Desulfovibrio strains have been found in a variety of habitats, including soil, the intestines and feces of animals, and both salinic and fresh water. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Desulfomonas pigra atcc 29098t, desulfovibrio desulfuricans subsp. Desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell il6 and il8 secretion and eselectin and vcam1 expression. A combined pathway of sulfur compound disproportionation in. By combining in vitro experiments with bioinformatic and rnaseq. Strain details desulfovibrio desulfuricans strain g20 is a spontaneously nalidixic acid resistant derivative of the wildtype strain g100a that was isolated from an oil well corrosion site weimer et al. The rcsb pdb also provides a variety of tools and resources.
By combining the uf with flocculation process, bacteria had been removed until. Pdf sulfatereducing bacterium desulfovibrio desulfuricans. Sulfate reducing bacteria srb 1 are a broad group of bacteria that have in common the fact that they derive energy from the respiration of sulfate. Pdf structure refinement of the aldehyde oxidoreductase. Biochimica et biophysica actaprotein structure and molecular enzymology 12051. M or nmolmg protein to cell suspensions incubated in 150 mm kcl at1c were almost completely taken up and accumulated about 5,000fold. Desulfovibrio desulfuricans strain g20, which was found at an oil well corrosion site 2can. Johns hospital health center, santa monica, california 90404 3. The two negativeion mass spectra gave a rough idea of the degree of heterogeneity present in the preparation. Sulfate reducing bacteria problems in water treatment. Dde i from desulfovibrio desulfuricans strain norway. Degradation of nitrocellulosebased paint by desulfovibrio desulfuricans atcc 541 l. Desulfovibrio desulfuricans isolates from the gut of a single individual.
Pdf upconversion of cellulosic waste into a potential drop in. Desulfovibrio desulfuricans also known as strain nd 2 description and significance. At ph 5, cell population densities decreased signi. Despite recent progress, the specific factors that cause or ameliorate these diseases largely remain to be identified. Degradation of nitrocellulosebased paint by desulfovibrio. Desulfovibrio desulfuricans strain atcc 27774 dsm 6949.
Lactate or h2 served as the electron donor and the oxidized crvi, movi, sevi and uvi served as electron acceptors. Desulfovibrio desulfuricans beijerinck 1895 kluyver and van niel 1936, 397al spirillum desulfuricans beijerinck 1895, 1. Desulfovibrio desulfuricans strain g20 is a spontaneously nalidixic acid resistant derivative of the wildtype strain g100a that was isolated from an oil well corrosion site weimer et al. Because of its metal corroding ability, which has consequently led to numerous health and safety concerns in industry, a means to neutralize and better understand desulfovibrio species is the goal of research on desulfovibrio itqb. The quality of the spectra is similar, and two peaks were common to both spectra at mz 60. Request pdf bioreduction and biocrystallization of palladium by desulfovibrio. It is generally found in soils, waters, and the stools of animals, although in rare cases it has been found to cause infection in humans.
As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. Corrosion of iron and formation of iron phosphide by. Kroneck fachbereich biologie, mathematischnaturwissenschaftliche sektion, universita. Desulfovibrio desulfuricans beijerinck, 1895 kluyver and van niel, 1936. Reduction of uranium by desulfovibrio desulfuricans. Identification of these species with conventional methods is difficult. Extracellular ironsulfur precipitates from growth of.
Intracellular hg0 oxidation in desulfovibrio desulfuricans. Desulfovibrio desulfuricans bacteremia in an immunocompromised. The reported case of a desulfovibrio desulfuricans. Information and translations of desulfovibrio in the most comprehensive dictionary definitions resource on the web. Sulfate reducing bacteria are responsible for significant corrosion of equipment. Desulfovibrio is a genus of gramnegative sulfatereducing bacteria.
However, under anaerobic conditions, small colonies of motile bacilli were observed 48 h after inoculation. Uptake of 35slabelled sulfate was studied with a new isolate of desulfovibrio desulfuricans, strain csn. Reduction of sulfur dioxide by desulfovibrio desulfuricans in co. The subspecies desulfovibrio desulfuricans desulfuricans was originally described by kluyver and van niel 1936.
Growth of desulfovibrio desulfuricans with sulfate, nitrate, or nitrite as the only electron acceptor. Eight of the 16 isolates 50% have been obtained from patients with appendicitis andor intraabdominal abscesses, and in six other cases. Alden research laboratory, 1 infectious diseases division, 2 microbiology laboratory, st. It has 907 residues on a single polypeptide chain, a. Gramnegative sulphatereducing bacterium desulfovibrio desulfuricans 29 30 31.
In this study, we conducted experiments with the hgmethylating bacterium desulfovibrio desulfuricans. The yield per mole fermented pyruvate is much lower than the yield per mole respired pyruvate. Direct spectroscopic evidence for the presence of a 6fe cluster in an ironsulfur protein isolated from desulfovibrio desulfuricans atcc 27774 published erratum appears in j. Bioreduction and biocrystallization of palladium by desulfovibrio. Isolated from a rumen fluid enrichment, this culture has been a model syntrophic partner due to its metabolic flexibility. Cappitelli a a dipartimento di scienze e tecnologie alimentari e microbiologiche, universita degli studi di milano, via celoria 2, 203 milan, italy. Get a printable copy pdf file of the complete article 1. Metalmining bacteria are green chemists sciencedaily. The ninehaem cytochrome c 9hcc, previously described as having 12 haem groups, was isolated from cells of desulfovibrio desulfuricans atcc 27774, grown under both nitrate and sulphaterespiring conditions. Jan 15, 2020 525146 desulfovibrio desulfuricans strain atcc 27774 dsm 6949 strain. Pdf the treatment of wellwater containing bacteria desulfovibrio desulfuricans used. The worlds first wiki where authorship really matters nature genetics, 2008. We propose the use of desulfovibrio desulfuricans nd2 as a model species for understanding the mechanism of microbial hg. Reduction of the oxidized metal species resulted in the precipitation of solid phases of the metals.
Beech1 8 9 1 school of pharmacy and biomedical sciences, university of portsmouth, st. Therefore, manipulation and growth of cultures should be performed in an anaerobic growth chamber coy laboratory product, inc. Effects of oxygen on the growth of desulfovibrio desulfuricans. The purification and properties of the hydrogenase of. Under anoxic conditions, certain anaerobic bacteria can oxidize dissolved elemental mercury and convert the oxidized hg to neurotoxic methylmercury. Threedimensional structure of the nonaheme cytochrome c. Desulfovibrio article about desulfovibrio by the free.